Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Folic Acid Size:100g

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Folic Acid Size:100gFolic acid is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Folic acid or to replace this vitamin. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room

3g in 1L of Distilled or De-ionised Water

1% Store at 2 - 8°C in dark

20 Suspend 47

3% Residue after Ignition <0

SD Agar / Galactose and Raffinose containing Yeast Nitrogen base without Amino Acids

Suspend 18

2 at 25°C Suspend 40 gram powdered medium in 1 litre distilled water

Inositol is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of inositol or to replace this vitamin

developed by Hanh

Amino Acids   Arg 700 Asp 300 Asp A 150 Cys 300 GluA 545 Gly 900 His 300 Ile 600 Leu 900 Lys 1250 Met 350 Phe 550 Pro 800 Thr 500 Trp 350 Val 700 Vitamins   Biotin 0

These variations in Sodium chloride content make it possible to select the optimal salt concentration for a specific strain

2 at 25°C Suspend 43